Generator

Part:BBa_I766255

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-25)

HMTM1-EE under strong inducible promoter

Usage and Biology

Construct is under control of pAdh, a strong constitutive promoter.

EE is one half of the EE-RR leucine zipper interaction, which has a dissociation constant in the nanomolar range.

HMTM1 stands for Human Myotubularin(MTM) related protein 1. HMTM1 binds Phosphatidylinositol 3-Phosphate (PI[3]P) and Phospatidylinosotol 3,5-Phosphate (PI[3,5]P) as substrates and dephosphorylates them at the 3-Phosphate position.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 1699
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 1352
    Illegal PstI site found at 2748
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 1699
    Illegal EcoRI site found at 2397
    Illegal PstI site found at 2748
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 1699
    Illegal EcoRI site found at 2397
    Illegal BglII site found at 1115
    Illegal BamHI site found at 3043
    Illegal XhoI site found at 1012
    Illegal XhoI site found at 1096
    Illegal XhoI site found at 1298
    Illegal XhoI site found at 2482
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 1699
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 1352
    Illegal PstI site found at 2748
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 1699
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 1352
    Illegal PstI site found at 2748
    Illegal AgeI site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None